Stem cells therapy in Dentistry

formazione medica

di Dr.Eugene Zadorin.PhD

Per la registrazione, contattare Dr.Eugene Zadorin, dottorato di Ricerca
All registered participants will receive certificates at the end of the course :
1) “Buona Pratica Clinica”
2) “Esperto in terapia con cellule staminali”

Giorno 1

10.00
introduzione
The stem cells are divided in two groups:
1) the embryonic stem cells and
2) the adult stem cells.
The latter are located in human tissues such as bone marrow, pelle, adipose tissue and dental pulp
Several populations of mesenchymal stem cells are found in the tooth.
These different cell types are categorized according to their location in the tooth and they demonstrate
slightly different features. It appears that the dental stem cells isolated from the dental pulp
and the periodontal ligament are the most powerful cells for tooth engineering.
Role of epithelial stem cells, which are important for the regeneration of the enamel.
La medicina rigenerativa sta attirando molta attenzione perché promette nuove cure terapeutiche
tecniche di terapia con cellule staminali per la riparazione e la sostituzione di tessuti e organi che hanno perso funzioni a causa dell'invecchiamento, malattia, danno, e difetti congeniti .
Le applicazioni cliniche sulle cellule staminali hanno già iniziato a riparare un'ampia varietà di tessuti, come il sangue, pelle, cornea, cartilagine, e osso.
L'odontoiatria ha da tempo abbracciato il ripristino della funzione dei tessuti e degli organi.
An example of Dental Regeneration is the dental implant only recently incorporating closure with stem cell based soft tissue. Stem cells found in teeth harvested during routine dental visits are cryopreserved and appear to replicate at a higher rate than those from other parts of the body.
12.00
Stem Cells from human exfoliated deciduous teeth (SHED)
Advantages of banking SHED cells includere: It’s a simple painless technique to isolate
them and being an autologous transplant they don’t possess any risk of immune reaction
or tissue rejection and hence immunosuppressive therapy is not required. SHED may also
be useful for close relatives of the donor such as grandparents, parents and siblings.
Apart from these, SHED banking is more economical when compared to cord blood and
may be complementary to cord cell banking.
The most important of all these cells are not subjected to same ethical concerns as embryonic stem cells.
13.00
Periodontal ligament stem cells (PDLSCs)
Multipotent postnatal stem cells in the human periodontal ligament (PDLSCs). When transplanted into rodents,
PDLSCs had the capacity to generate a cementum/periodontal ligament-like structure and contributed
to periodontal tissue repair. These cells can also be isolated from cryopreserved periodontal ligaments
while retaining their stem cell characteristics, including single-colony strain generation,
cementum/periodontal-ligament-like tissue regeneration, expression of MSC surface markers,
multipotential differentiation and hence providing a ready source of MSCs.
13.30
Stem cells from apical papilla (SCAP)
MSCs residing in the apical papilla of permanent teeth with immature roots are known as SCAP.
SCAP are capable of forming odontoblast-like cells,
producing dentin in vivo, and are likely cell source of primary odontoblasts for the formation of root dentin.
SCAP supports apexogenesis, which can occur in infected immature permanent teeth with periradicular periodontitis
or abscess. SCAP residing in the apical papilla survive such pulp necrosis because of their proximity to the periapical tissue vasculature.
14.00 Rompere
14.30
APPLICATIONS IN DENTISTRY
Most research is directed toward regeneration of damaged dentin, pulp, resorbed root,
periodontal regeneration and repair perforations. Whole tooth regeneration to replace the traditional dental implants is also in pipeline.
Tissue-engineering applications using dental stem cells that may promote more rapid healing
of oral wounds and ulcers as well as the use of gene-transfer methods to manipulate salivary proteins and oral microbial colonization
In generale,autologous bone regeneration in humans with the use of DP Stem cells in collagen sponge demonstrates optimal repair of bone defects of the mandible and the restoration of periodontal tissue
Adult dental stem cells can differentiate into many dental components, such as dentin, periodontal ligament,
cement and dental pulp tissue, but not into enamel.
The stem cells also produce a host of beneficial neurotropic factors such as glial cell lined neurotropic factor, nerve growth, and brain derived neurotropic factor, potentially promoting the survival of brain neurons lost in neuro degenerative diseases such as Parkinson’s disease.
Injecting dental stem cells into the basal ganglia can potentially provide neurotropic support for dying nerve cells and replacement of dead cells.
Intravenous injections of stem cells derived from human exfoliated deciduous tooth pulp (SHED) have a protective effect against brain damage ,ictus.
Image result for dentistry stem cells
Cells extracted from dental pulp have proven to be more successfully banked and preserved than those from other sources in the body. By comparison they are more available and accessible as anticipated regenerative medical breakthroughs occur.
16.30
The use of stem cells in osseous regeneration
Adult MSCs recently identified in the gingival connective tissues (gingival mesenchymal stem cells [GMSCs]) have osteogenic potential and are capable of bone regeneration in mandibular defects.
I GMSC sopprimono anche la risposta infiammatoria inibendo la proliferazione dei linfociti e le citochine infiammatorie e promuovendo il reclutamento di cellule T regolatorie e citochine antinfiammatorie.
così, I GMSC potenzialmente promuovono l'ambiente "giusto" per la rigenerazione ossea e viene attualmente esplorato terapeuticamente.
Per la registrazione, contattare Dr.Eugene Zadorin, dottorato di Ricerca
Giorno 2
10.00
PROSPETTIVE DI CELLULE STAMINALI DENTALI IN MEDICINA
Cellule staminali dentali per applicazioni mediche come terapie cardiache,
tessuto cerebrale rigenerante, per le terapie della distrofia muscolare e per la rigenerazione ossea.
SHED can be used to generate cartilageas well as adipose tissue.
Bone grafting for reconstruction of large size cranial bone defects
Stem cells offer a new perspective targeting dental pulp regeneration and further development of root structure. Both SHED and DPSC can differentiate into odontoblasts
11.00
Stem cell therapies is Periodontology.
Other areas that may benefit from the development of stem cell therapies is Periodontology.
regeneration with stem cells or/and bioabsorbable matrices are the main therapies used to regenerate the periodontium tissues
12.00
Induced stem cells from harvested human dental stem cells
Our innovative method is to generate induced stem cells from harvested human dental stem cells.
Shinya Yamanaka has been recently awarded The Nobel Prize in Physiology or Medicine 2012 for generating iPSC
This approach reprograms dental stem cells into an embryonic state, thus expanding their potential to differentiate into a much wider range of tissue types.
Our stem cells lab succeeded in making specific dental tissues or tooth like structures
for regeneration of functional tooth in humans.
Dental Pulp Stem Cells into muscle, nerve/spinal cord, osso, cuore, fegato, cervello, pelle (epithelial), ligaments & cartilagine
13.00
Banking stem cells
Obtaining and banking stem cells is an expanding business.
Our biotechnology stem cells Laboratory offer the whole package of collecting and storing dental stem cells
for a lower cost as compared to cord blood stem cells.
To maintain cell viability, collection kits are prepared to keep cells alive and shelter freshly extracted teeth from temperature changes.
Even with these precautions, the time passed from gathering to arrival at the processing laboratory must be shorter than 72 h
Image result for dentistry stem cells
14.00
Rompere
14.30
Dental stem cell banking
Dental stem cell banking services .
Dental Stem Cells / Cellule staminali mesenchimali (MSC) are harvested from the teeth
Stem cell therapy is the new realm of regenerative medicine for Diabetes Type 1, Wound Healing, Parkinson, eccetera.
15.00
Stem cell storage.
The approaches used for stem cell storage are:
(un') Cryopreservation (B) magnetic freezing.
15.15
Cryopreservation
It is the process of preserving cells or whole tissues by cooling them to sub-zero temperatures.
Cells harvested near end of log phase growth (circa. 80–90% confluent) are best for cryopreservation.
Liquid nitrogen vapour is used to preserve cells at a temperature of <−150°C.
In a vial 1.5 ml of freezing medium is optimum for 1–2 × 106 cellule.
16.00
Magnetic freezing
This technology is referred to as cells alive system (CAS), which works on principle of applying
a weak magnetic field to water or cell tissue which will lower the freezing point of that body by up to 6–7°C.
it can increase
the cell survival rate in teeth to 83%.
CAS system is a lot cheaper than cryogenics and more reliable.
Per la registrazione, contattare Dr.Eugene Zadorin, dottorato di Ricerca
All registered participants will receive certificates at the end of the course :
1) “Buona Pratica Clinica”
2) “Esperto in terapia con cellule staminali”

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terapia con cellule staminali