Última actualización: Agosto 17, 2026
Can mesenchymal stem cells induce tolerance to cotransplanted human embryonic stem cells?
Puymirat E1, Geha R, Tomescot A, Bellamy V, Larghero J, Trinquart L, Bruneval P, Desnos M, Hagège A, Pucéat M, Menasche P..
Células madre mesenquimales (MSC) are reported to be immune privileged. We assessed whether their transplantation (Tx) could create a suppressive microenvironment mitigating rejection of coinjected human embryonic stem cells (hESC).
Three weeks after ligation-induced myocardial infarction, 40 immunocompetent rats received 150 microl of cardiac-specified hESCs (5 incógnita 10(6)), MSC (5 incógnita 10(6)), hESC + MSC (5 incógnita 10(6) for each), or control medium. Two months after Tx, ventrículo izquierdo (LV) function was assessed by echocardiography, and hearts were processed for the detection of human cells by immunostaining and quantitative RT-PCR, patterns of rejection, fibrosis, y angiogénesis. Two months after Tx, LV ejection fraction (FEVI) was significantly higher in the ESC and ESC + MSC groups compared with controls. There were few engrafted cells, which expressed markers of endothelial, músculo liso, and ventricular cardiac cells, particularly in the MSC group.
Hearts of all groups demonstrated a similar infiltration by CD4(+) and CD3(+) cells but MSC-Tx resulted in a greater infiltration of FoxP3 compared with the control and ESC-alone groups. No teratoma was observed. De este modo, cotransplantation of ESCs and MSCs provided better functional preservation compared with single-cell treatment alone. Sin embargo, there was only modest evidence for an immunosuppressive effect of coinjected MSCs and their beneficial effects seemed rather mediated by trophic effects on the host tissue.
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