间充质干细胞能否诱导人胚胎干细胞共移植的耐受性?
普米拉特 E1, 格哈R, 托梅斯科特, 贝拉米五世, 拉盖罗·J, 特林夸特l, 布鲁内瓦尔·P, 德斯诺斯米, 哈热格A, 普塞特·M, 梅纳谢普.

间充质干细胞 (间充质干细胞) 据报道具有免疫特权. 我们评估了他们的移植是否 (发射机) could create a suppressive microenvironment mitigating rejection of coinjected human embryonic stem cells (人胚胎干细胞).

Three weeks after ligation-induced myocardial infarction, 40 immunocompetent rats received 150 microl of cardiac-specified hESCs (5 x 10(6)), 间充质干细胞 (5 x 10(6)), hESC + MSC (5 x 10(6) for each), or control medium. Two months after Tx, left ventricle (LV) function was assessed by echocardiography, and hearts were processed for the detection of human cells by immunostaining and quantitative RT-PCR, patterns of rejection, 纤维化, 和血管生成. Two months after Tx, LV ejection fraction (左心室射血分数) was significantly higher in the ESC and ESC + MSC groups compared with controls. There were few engrafted cells, which expressed markers of endothelial, 平滑肌, and ventricular cardiac cells, particularly in the MSC group.

Hearts of all groups demonstrated a similar infiltration by CD4(+) and CD3(+) cells but MSC-Tx resulted in a greater infiltration of FoxP3 compared with the control and ESC-alone groups. No teratoma was observed. 因此, cotransplantation of ESCs and MSCs provided better functional preservation compared with single-cell treatment alone. 然而, there was only modest evidence for an immunosuppressive effect of coinjected MSCs and their beneficial effects seemed rather mediated by trophic effects on the host tissue.