使用乌克兰骨髓干细胞

 

Bone marrow cells reside deep inside bone cavities in
the most protected part of the body and are redundant
throughout the organism. This preferential status reflects
the primary role these cells play in the survival of the
organism. Stem cells from marrow naturally home to,
thrive and proliferate in hypoxic tissue.

(10) In response to
外伤, which creates a hypoxic environment, marrow
cells mobilize into the vasculature from the medullary
space where the cells aggregate themselves into the
recently created hypoxic damaged tissue.

(3-10) 一旦
居民, marrow stem cells are capable of functioning in
the hostile hypoxic environment to orchestrate the tissue
regeneration process. (10)

By capturing all of the nucleated cells through a proper
marrow aspiration, you are maintaining the same relative
proportion of cells that naturally aggregates at the defect
site. The following quote captures the essence of this
insight “These data demonstrate that removing BMSC’s
(bone marrow stem cells) from their normal environment
of complimentary cells reduces their osteoblastic capacity
and that to achieve their maximal dierentiation, BMSC’s
require direct physical contact with accessory cells.”

(2)
所以, the potential of marrow-sourced
nucleated cells should be thought of as a group of
dierent cells that is able to:

1) Home to and self organize at the defect site
2) Release the appropriate levels of various growth
factors to influence the function and cytokine production
of resident cells based on the stage of the healing
cascade and

3) Cooperate with and influence resident cells to
accomplish the steps of the healing cascade that
culminates in repair. (1,10, 19-38)
Each nucleated cell type contributes to the process of
组织修复. (2)

例如, granulocytes in the area of
bone regeneration release large amounts of VEFG which
up regulates the production of BMP-2 and BMP-6 by
sub-populations of CD-34 + 细胞. (37,38)

Properly aspirating marrow, and appropriately
administering the cells is significantly enhancing and
exactly mimicking the body’s natural healing process. (10)
This simple explanation is fundamental to the use of
marrow cells in damaged tissue. In the case of bone
grafting, the cells need to be combined with a functional
bone matrix. In the case of osteonecrosis or heart
disease and limb ischemia, the ischemic bone or muscle
serves as the scaold and the cells are directly injected
to the site. (14,16,30) Trauma that does not heal is often
the result of an inadequate number of mobilized marrow
cells at the site of the defect. (11,12)

Autograft exactly mimics and supplements the natural
process in that resident in the harvested bone are the
living cells of the marrow that are cut from one part of the
body and transplanted to another. Autograft is especially
suited for bone repair as the bone from the autograft
serves as an appropriate scaold for the transplanted cells.
事实上, by collagenase digesting human autograft and
counting the cells, Muechler et al demonstrated that
autograft has approximately the number of nucleated
cells per CC and cfu-f as the Marrow CellutionTM needle
aspirate. (29,42) By combining the nucleated cell rich
fraction with a functioning matrix, you create the same
treating composition, (types and ratio of cells) as is
contained in autograft without the morbidity.

By examining all of the cellular components of autograft,
we see that those same cells are contained marrow
aspirate. (29) When we look at the role of those dierent
cells in bone formation, we see that each of the cellular
components of autograft, and thus by extension, marrow,
play a positive role in the micro-environment of bone
formation. 例如, T-cells and other lymphocytes
improve the mobility and functionality of EPC’s (39, 40) 在
addition; some of the most potent stem cells reside in the
deeper granulocytic layer. (28) This is likely because
cycling progenitor cells increase the density of their
nucleus just before going through mitosis. 所以, capturing
all of the nucleated cells, similar to autograft, 是个
optimal strategy.

A review of the literature demonstrates
similar clinical outcomes to autograft when using high
concentrations of nucleated cells from marrow as
measured by cfu-f. 例如, Hernigou et al in
non-union, Gan et al in multilevel lumbar spine fusion,
Marrow CellutionTM and

A Functional Bone Matrix:
The Power of Synergy Jager et al in orthopedic bone defects, Gangji et al and
Herigou et al in osteonecrosis,Velardi et al for pediatric
skull defects, and Sauerbier et al for sinus lift
augmentation. (13-18,35)
Each cell in a dierent micro-environment can provide a
dierent function; 例如, granulocytes are
inflammatory in the micro-environment of infection
compared to pro-regeneration in the micro-environment
of building new bone or tissue. (1, 41) Just because a cell
has what could be construed to be a negative impact
such as pro-inflammatory in a dierent
micro-environment( ie granulocytes in an area of
感染), does not mean that cell will have that impact in
another micro-environment such as tissue repair. Recent
insights has focused on the role that marrow stem cells
play in regulating other cells during the immune
reparative processes at the sight of trauma.

(43)
A marrow aspirate that
is ecient at capturing
nucleated cells has the
same number and
types of nucleated
cells as autograft.
Bone marrow cells reside deep inside bone cavities in
the most protected part of the body and are redundant
throughout the organism. This preferential status reflects
the primary role these cells play in the survival of the
organism. Stem cells from marrow naturally home to,
thrive and proliferate in hypoxic tissue. (10) In response to
外伤, which creates a hypoxic environment, marrow
cells mobilize into the vasculature from the medullary
space where the cells aggregate themselves into the
recently created hypoxic damaged tissue. (3-10) 一旦
居民, marrow stem cells are capable of functioning in
the hostile hypoxic environment to orchestrate the tissue
regeneration process. (10)

By capturing all of the nucleated cells through a proper
marrow aspiration, you are maintaining the same relative
proportion of cells that naturally aggregates at the defect
site. The following quote captures the essence of this
insight “These data demonstrate that removing BMSC’s
(bone marrow stem cells) from their normal environment
of complimentary cells reduces their osteoblastic capacity
and that to achieve their maximal dierentiation, BMSC’s
require direct physical contact with accessory cells.” (2)
所以, the potential of marrow-sourced
nucleated cells should be thought of as a group of
dierent cells that is able to:

1) Home to and self organize at the defect site
2) Release the appropriate levels of various growth
factors to influence the function and cytokine production
of resident cells based on the stage of the healing
cascade and

3) Cooperate with and influence resident cells to
accomplish the steps of the healing cascade that
culminates in repair. (1,10, 19-38)
Each nucleated cell type contributes to the process of
组织修复. (2) 例如, granulocytes in the area of
bone regeneration release large amounts of VEFG which
up regulates the production of BMP-2 and BMP-6 by
sub-populations of CD-34 + 细胞. (37,38)
Properly aspirating marrow, and appropriately
administering the cells is significantly enhancing and
exactly mimicking the body’s natural healing process. (10)
This simple explanation is fundamental to the use of
marrow cells in damaged tissue. In the case of bone
grafting, the cells need to be combined with a functional
bone matrix. In the case of osteonecrosis or heart
disease and limb ischemia, the ischemic bone or muscle
serves as the scaold and the cells are directly injected
to the site. (14,16,30) Trauma that does not heal is often
the result of an inadequate number of mobilized marrow
cells at the site of the defect. (11,12)
Autograft exactly mimics and supplements the natural
process in that resident in the harvested bone are the
living cells of the marrow that are cut from one part of the
body and transplanted to another. Autograft is especially
suited for bone repair as the bone from the autograft
serves as an appropriate scaold for the transplanted cells.
事实上, by collagenase digesting human autograft and
counting the cells, Muechler et al demonstrated that
autograft has approximately the number of nucleated
cells per CC and cfu-f as the Marrow CellutionTM needle
aspirate. (29,42) By combining the nucleated cell rich
fraction with a functioning matrix, you create the same
treating composition, (types and ratio of cells) as is
contained in autograft without the morbidity.
By examining all of the cellular components of autograft,
we see that those same cells are contained marrow
aspirate. (29) When we look at the role of those dierent
cells in bone formation, we see that each of the cellular
components of autograft, and thus by extension, marrow,
play a positive role in the micro-environment of bone
formation. 例如, T-cells and other lymphocytes
improve the mobility and functionality of EPC’s (39, 40) 在
addition; some of the most potent stem cells reside in the
deeper granulocytic layer. (28) This is likely because
cycling progenitor cells increase the density of their
nucleus just before going through mitosis. 所以, capturing
all of the nucleated cells, similar to autograft, 是个
optimal strategy. A review of the literature demonstrates
similar clinical outcomes to autograft when using high
concentrations of nucleated cells from marrow as
measured by cfu-f. 例如, Hernigou et al in
non-union, Gan et al in multilevel lumbar spine fusion,
Jager et al in orthopedic bone defects, Gangji et al and
Herigou et al in osteonecrosis,Velardi et al for pediatric
skull defects, and Sauerbier et al for sinus lift
augmentation. (13-18,35)
Each cell in a dierent micro-environment can provide a
dierent function; 例如, granulocytes are
inflammatory in the micro-environment of infection
compared to pro-regeneration in the micro-environment
of building new bone or tissue. (1, 41) Just because a cell
has what could be construed to be a negative impact
such as pro-inflammatory in a dierent
micro-environment( ie granulocytes in an area of
感染), does not mean that cell will have that impact in
another micro-environment such as tissue repair. Recent
insights has focused on the role that marrow stem cells
play in regulating other cells during the immune
reparative processes at the sight of trauma. (43)

Aspirating high quality marrow and
combining it with a functioning matrix
for transplantation into a bone defect is
exactly mimicking and supplementing
the body’s natural response to that
surgical trauma site. Clinical results are
positively correlated to the number of
cfu-f contained in the graft. (15-18,35)

1

 

来源:

 

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105, 没有. 3, pp. 1068-1077
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Otsuru et al Stem Cells 2008;26 223-234
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Sebastian Sauerbier, Rainer Schmelzeisen, Ralf Gutwald, Journal of CranioMaxillofacial Surgery, 补充, 2008, 卷. 36(-):158
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stem cells in tissue regeneration” Bone Marrow Transplantation 2009 43 69-81
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概念,Alternative Hypothesis, and Relationship to the Bone Remodeling
Compartment” : Journal of Cellular Biochemistry 103: 393-400 ; 2008
21 Hematopoietic Stem Cells Regulate Mesenchymal Stromal Cell Induction into
Osteoblasts Thereby Participating in the Formation of the Stem Cell Niche Stem
Cells Younghun Junga Vol. 26 没有. 8 八月 2008, pp. 2042 –2051
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Bone Fracture” Mifune et al Stem Cells 2008; 26:1395-1405
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